Fertility and flow cytometry study of frozen-thawed sperm in cryopreservation medium supplemented with soybean lecithin
نویسندگان
چکیده
منابع مشابه
P-146: Fertility and Flow Cytometric Evaluations of Frozen-Thawed Rooster Semen in Cryopreservation Medium Containing Low Density Lipoprotein
Background: Frozen-thawed rooster semen is not reliable for use in artificial insemination in commercial stocks. Low density lipoprotein (LDL) has been assessed for effectiveness as a cryoprotectant in the extender to improve the quality of frozen-thawed rooster semen. Although LDL has been evaluated in a few studies in other species for semen cryopreservation, to date, no study has been conduc...
متن کاملEffects on glycocalyx structures of frozen-thawed bovine sperm induced by flow cytometry and artificial capacitation
Sperm sorting by flow cytometry is a useful technology in the bovine industry, but the conception rates after artificial insemination using sex-sorted sperm are lower than when using the un-sorted sperm. In this study, we have investigated the causes for these low conception rates. We have focused on changes caused by flow cytometry to the glycocalyx, which forms the outermost surface of the sp...
متن کاملIn vitro fertilisation with frozen-thawed bovine sperm sexed by flow cytometry and validated for accuracy by real-time PCR.
The methodologies used for cytometric sorting of fresh spermatozoa never allowed a clear resolution of sexual chromosomes of frozen-thawed semen. To devise a novel method for the production of bovine predefined sexed embryos using frozen-thawed semen, sorting efficiency of different protocols was studied using a new quantitative real-time PCR method to verify the purity of sexed semen. To this ...
متن کاملP-127: In Vitro Assessment of Trehalose for Cryopreservation of Ram Sperm in Extender Containing Soybean Lecithin
Background: Trehalose, a non-permeable disaccharide, has a protective role related both to osmotic effect and specific interactions with membrane phospholipids, rendering hypertonic media, causing cellular osmotic dehydration before freezing, and then decreasing the amount of cell injury by ice crystallization. Within this study, an attempt was made to evaluation of two levels of 50 and 100 mM ...
متن کاملComparisons between Two Antioxidant Butylated Hydroxytoluene and Glutathione Supplemented Cryopreservation Medium on Human Sperm DNA Integrity
Introduction: Cryopreservation of semen is routinely used in a variety of circumstances including before assisted reproduction treatments, pre- radiation or chemotherapy treatment and etc. The aim of this study was to compare the effect of Butylated hydroxytoluene (BHT) and Glutathione supplemented cryopreservation medium on sperm parameters and amount of DNA fragmentation during the freeze-tha...
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ژورنال
عنوان ژورنال: Cryobiology
سال: 2016
ISSN: 0011-2240
DOI: 10.1016/j.cryobiol.2016.05.010